Manufacturing internal control (AFB) smears by PhAS and BAS treated sputum samples

SJ VINCY, C MATHIALAGAN - SALT Journal of Scientific Research in …, 2021 - pkp.saltjsrh.in
SJ VINCY, C MATHIALAGAN
SALT Journal of Scientific Research in Healthcare, 2021pkp.saltjsrh.in
Objective: Internal control smears were prepared using phenol ammonium sulfate (PhAS)
and bleach ammonium sulfate (BAS) methods. Methods: A complete of 150 smears were
prepared, 80 smears were stained, and two different technologists validated 60 smears.
Results: Consistency was found to be true when compared with the quality consistency table
for all grades in both methods, and M±2SD was within the boundaries. Conclusion: This
study suggests that PhAS and BAS are alternate concentration methods for the preparation …
Abstract
Objective: Internal control smears were prepared using phenol ammonium sulfate (PhAS) and bleach ammonium sulfate (BAS) methods. Methods: A complete of 150 smears were prepared, 80 smears were stained, and two different technologists validated 60 smears. Results: Consistency was found to be true when compared with the quality consistency table for all grades in both methods, and M±2SD was within the boundaries. Conclusion: This study suggests that PhAS and BAS are alternate concentration methods for the preparation of internal control smears. Keywords: Internal control, PhAS, BAS, sedimentation, grade suspension
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